Question

2. The results of the RNA sequencing experiments are raw transcript reads with no sequence identification. The sequence reads correspond to various genes.

A. Describe how you would identify unknown sequence reads.

B. How would you amplify and isolate just one of the target sequences from the RNA sequencing experiment? Include both computer and wet lab methods you would use to identify and isolate a single target gene.

Answer 1

A.

Transcriptome reconstruction is the technology that is used to identify all the transcripts that expressed in an specimen. This is done by read mapping data. The information content of any organism usually recorded in the DNA of its genome and then used to express through the transcription. Transient intermediary molecule is served by mRNA in the information network.

B.

To amplify only one of the target sequence, we will be required a primer specific to the given sequence. The specific primer to desired sequence will help in amplifying the specific sequence. This sequence can be isolated by choosing specific restriction enzymes.