Question

AAACAAAATCCTAACCACGTTTGGTGGGAAGTATCTGCCCACGTT TTTGTTTTAGGATTGGTGCAAACCACCCTT CATAGACGGGTGCAA From the two complementary strands, identify the coding strand and the template strand...

AAACAAAATCCTAACCACGTTTGGTGGGAAGTATCTGCCCACGTT

TTTGTTTTAGGATTGGTGCAAACCACCCTT CATAGACGGGTGCAA

From the two complementary strands, identify the coding strand and the template strand with polarity labeling.
Find the start codon in the open-reading frame in a 5' to 3' direction. Find the stop codon in the 5' to 3' direction.
Does this strand belong to a prokaryotic or eukaryotic organism?
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Homework Answers

Answer #1
  1. Before starting to answer the question few points to revise:
  2. Template strand runs from 3' to 5'
  3. Coding strand runs from 5'-3'
  4. Thymine is present in DNA
  5. For eukaryotes,
  • start codon: ATG
  • stop codon : TGA / TAA / TAG

Below given is the two strands given in the question:

3'- AAACAAAATCCTAACCACGTTTGGTGGGAAGTATCTGCCCACGTT -5' ---------> TEMPLATE STRAND

stop codon start codon

5'- TTTGTTTTAGGATTGGTGCAAACCACCCTTCATAGACGGGTGCAA-3' ---------> CODING STRAND

  • Since thymine is present the strands definitely belong to a eukaryotic organism since thymine is present in DNA (not in RNA) and eukaryotic organisms have DNA
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