Question

A full plate of PCR tubes contains 96 wells, each of which can have its own reaction. Determine how much GoTaq Master Mix you ill need to add to your PCR mix, if each reaction requires 12.5 uL and you add on 20% to account for the loss of reagent to plastics. How do you do it?

Answer 1

The procedure to calculate the amount of GoTaq Master Mix required is as follows :

For 96 wells, total quantity required = 96 x 12.5

= 1200 uL

To account for 20% loss of reagent to plastics, we need to compensate it by adding 20% extra GoTaq Master Mix.

So 20% of 1200 uL = (20/100) x 1200

= 0.2 x 1200

= 240 uL

Therefore, actual amount of GoTaq Master Mix required for 96 wells is given by

Initial Amount + Lost Amount

= 1200 + 240

= 1440 uL

So 1440 uL of GoTaq Master Mix will be required for PCR reaction.

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