Question

Absorbance Values for BSA Standard Curve
BSA Concentration (mg/mL)	Absorbance Trial 1	Absorbance Trial 2	Absorbance Trial 3	Absorbance Trial 4	Absorbance Trial 5	Absorbance Trial 6
0.2	0.239	0.247	0.195	0.234	0.287	0.280
0.4	0.510	0.499	0.513	0.522	0.586	0.549
0.6	0.796	0.771	0.695	0.754	0.799	0.800
0.8	1.045	1.002	0.999	1.023	1.015	1.018
1.0	1.260	1.197	1.045	1.200	1.243	1.107

Absorbance Values for IgG Standard Curve
IgG Concentration (mg/mL)	Absorbance Trial 1	Absorbance Trial 2	Absorbance Trial 3	Absorbance Trial 4	Absorbance Trial 5	Absorbance Trial 6
0.2	0.135	0.122	0.105	0.117	0.142	0.118
0.4	0.317	0.300	0.335	0.314	0.323	0.295
0.6	0.502	0.488	0.485	0.472	0.503	0.402
0.8	0.668	0.650	0.677	0.701	0.690	0.628
1.0	0.865	0.806	0.824	0.914	0.853	0.822

Absorbance Values for BSA and IgG (0.5 mg/mL) Samples
Sample	Absorbance Trial 1	Absorbance Trial 2	Absorbance Trial 3	Absorbance Trial 4	Absorbance Trial 5	Absorbance Trial 6
BSA (0.5 mg/mL)	0.624	0.652	0.602	0.620	0.621	0.630
IgG (0.5 mg/mL)	0.394	0.390	0.395	0.382	0.385	0.374

Absorbance Values for Drink Samples
Sample	Absorbance Trial 1	Absorbance Trial 2	Absorbance Trial 3	Absorbance Trial 4	Absorbance Trial 5	Absorbance Trial 6
1	0.673	0.652	0.689	0.649	0.701	0.755
2	0.802	0.783	0.757	0.797	0.706	0.757
3	0.714	0.782	0.738	0.769	0.773	0.729
4	0.615	0.578	0.545	0.706	0.601	0.612
5	0.421	0.495	0.458	0.417	0.485	0.489

Information for Drink Samples
Sample	Description	Amount of Protein per serving size	Dilution Factor Used in Assay
1	Neilson 2% milk	9 g per 240 mL	50
2	So Nice Organic Almond	1 g per 250 mL	5
3	Natura Rice Organic Original	2 g per 250 mL	10
4	Organic Soy Milk	8 g per 250 mL	50
5	Boost Instant Breakfast	15 g per 280 mL	100

ONE figure of the standard curves. Both BSA and IgG curves should be presented in one graph. Only the standard curve absorbance values are to be plotted. The absorbance values associated with the BSA0.5 sample and beverage samples should not appear on your standard curves.

- Plot only the mean absorbance values. - Show the +/- y-axis standard deviation error bars.

- Even though this is a graph, it must be called a “Figure” in the title. The figure is numbered as well. - The title is placed underneath the graph

. (ii) ONE table listing the mean protein concentration (in mg/mL) and standard deviation for: - The 0.5 mg/mL BSA and IgG samples as calculated using the BSA standard curve - The 0.5 mg/mL BSA and IgG samples as calculated using the IgG standard curve - The table title should be placed above the table and is numbered.

(iii) ONE table listing the mean protein concentrations for each beverage sample as calculated from both BSA and the IgG standard curve slopes. Remember, each sample was assayed a total of six times. Don’t forget to include the dilution factor associated with each sample in your concentration calculations. An example table is provided below for you to follow. The required information for this table is: - The mean protein for each beverage sample (in mg/mL) calculated from the BSA slope and show the value converted to g/serving size as given by the manufacturer in mL - The mean protein for each beverage sample (in mg/mL) calculated from the IgG slope and show the value converted to g/serving size as given by the manufacturer in mL - Don’t forget to clearly indicate the names of the samples and the published protein content per serving and in mg/mL.

Answer 1

Answer i. The mean and standard deviation of the absorbance value of the six trails for both BSA and IgG was computed. Fgiure (1) below shows the standard curve for both BSA and IgG (concentration versus absorbance). The points are the mean absorbnace values. The standard curves are almost linear and has postive trends. With increase in the concentration of the molecules, there is incresase in the absorbnace. BSA has comparative higher absorbnace than the IgG. The error bars shown in the figure is the standard deviation within the trials.