Question

We discussed the endosome system that viruses sometimes use to get their genetic material into the host cytoplasm.

A. Imagine an endosome that maintains a pH of 5 under normal conditions when no hydroxychloroquine is present. Explain how an endocytic pump could use ATP to create such an acidic pH.

B.Now imagine that hydroxychloroquine is suddenly present in the endosome. Explain the process that will happen to the hyroxychloroquine, the endosome pH, and the endocytic pump?

Answer 1

A. . They become increasingly acidic mainly through the activity of the V-ATPase. To maintain their acidic internal pH, lysosomes must actively concentrate H+ ions (protons). This is accomplished by a proton pump in the lysosomal membrane, which actively transports protons into the lysosome from the cytosol. This pumping requires expenditure of energy in the form of ATP hydrolysis, since it maintains approximately a hundredfold higher H+ concentration inside the lysosome.

B.  hyroxychloroquine inhibits the acidification of endosome. it causes alkaline pH

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