Question

Circular DNA can be cleaved by: Group of answer choices endonucleases and exonucleases, but not topoisomerases...

Circular DNA can be cleaved by:

Group of answer choices

endonucleases and exonucleases, but not topoisomerases

exonucleases, but not endonucleases or topoisomerases

endonucleases, topoisomerases, and exonucleases

endonucleases and topoisomerases, but not exonucleases

topoisomerases and exonucleases, but not endonucleases

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Answer #1

Ans : Circular DNA can be cleaved by

Endonucleases & topoisomerases but not exonucleases

Topoisomerases are responsible for preventing the supercoiling or torsional strain of double stranded DNA, which helps in stabilizing the DNA & helping the endonucleases to cut the specific recognition sites within the strand of DNA. So , endonucleases in association with topoisomerases helps in cleaving the circular DNA.

- But exonucleases plays no role in cleavage of circular DNA , as exonucleases cuts the linear double stranded DNA . These enzymes removes individual nucleotides from the ends of the linear strands.

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