Question

You are working in a tissue engineering lab and it is your goal to generate some liver tissue to transplant into a patient with the hope that it will continue to regenerate further once transplanted. Please tell me the following: What 3 things will you assemble to begin engineering your liver tissue? What would be the best source of cells for your work? What tests will you perform to ensure that the cells that are growing are becoming tissue cells? Please pick a specific protein you will check for AND step by step the assay you will use to see if the cells are expressing that protein.

Answer 1

The best source of cells for tissues engineering is embryonic stem cells, bone marrow derived mesenchymal stem cells, cord derived mesenchymal stem cells.

For the successful tissue engineering to be done, appropriate growth delivery system, sources of cells for the development of tissue, injectable orzonatec scaffolds are required.

In tissue engineering, using scaffolds is the technique; it utilises primary cells taken from patients and used in conjunction with scaffolds to form tissues for re-implantation. Scaffolds is used as cell support device upon which the cells are seeded in vitro, and that will be the foundation of tissue for transplantation. Second method is using scaffolds as growth factor, scaffolds are being combined with growth factors, so lump implantation cells from the body are recruited to the scaffolds sites and that will form tissues.