Question

To subculture cells, you must plate the cells on a new plates at a specific density that depends on that cell line and the purpose of your experiment. If you needed to plate the cells(ie. That your group resuspended in 50microliters) at a concentration of 2200 cells per cm2 onto 35mm plates that have an effective growth surface area of 9cm2, how would you do this? The final volume of media for a 35mm plate is 2mL.

A.Total # of 35mm culture dishes plated?

B. Volume of media to add to cell suspension?

Answer 1