Question

1. Euchromatin is associated with more loose and open organization of DNA and genes in these regions are more likely to be active and transcribed.

      
A. True

       
B. False

2. What is the role of gRNA in CRISPR?

      
A. It helps the cell recognizes that DNA is damaged and tries to repair it

       
B. It ensures that the Cas9 enzyme cuts at the right point in the genome

       
C. It recruits a cell's s own DNA repair enzymes to introduce changes to one or more genes

       
D. It makes a cut across two strands in the DNA

3. How is genetic information transferred during conjugation?

      
A. with the help of a virus

       
B. bidirectionally between two cells

       
C. from one bacterium to another

       
D. from one cell into the culture medium, where it is taken up by another cell

Answer 1

Ans 1: True. Euchromatin contains less tightly coiled chromosomes and most active genome, although it contains less DNA but it is early replicative and is readily available for transcription.

Ans 2: It ensures that the Cas9 enzyme cuts at the right point in the genome. gRNA or guide RNA helps in inserting and deleting residues in RNA editing process. It binds the Cas9 enzyme and then guides it to the specific location in the DNA where the Cas9 enzyme cuts the target DNA enzyme.

Ans 3: from one bacterium to another. Bacterial conjugation happens by direct cell to cell contact where one cell acts as donor while the other as recipient, it is a type of horizontal gene transfer.