Question

1. Transcribe and translate the normal sequence and the mutated sequence.
2. Identify what type of DNA mutation occurred. (insertion, deletion, or substitution)
3. Identify what type of protein change resulted. (frameshift, missense, nonsense, or silent)

normal sequence- 3' TAC GGA GCA GGG CCA AAA AAC TGT TTT ACT 5´

mutated sequence- 3´ TAC GGA GCA GGG CCA CCA AAC TGT TTT ACT 5´

Answer 1

A. Normal sequence

mRNA- 5' AUG CCU CGU CCC GGU UUU UUG ACA AAA UGA 3'

Protein- Met Pro Arg Pro Gly Phe Leu Thr Lys

Mutated sequence

mRNA- 5' AUG CCU CGU CCC GGU GGU UUG ACA AAA UGA 3'

Protein- Met Pro Arg Pro Gly Gly Leu Thr Lys

B. Substitution. The 3'AAA5' of normal sequence is substituted by 3'CCA5' in mutated sequence.

C. Missense mutation. A change in the protein sequence by replacement of one amino acid by a different amino acid is called missense mutation. Here, Phe is replaced by Gly.

Thanks