Question

A non-template DNA strand has the sequence 5’ -ACGTACAGTCCGT -3’. What is the sequence of ′′the...

  1. A non-template DNA strand has the sequence 5’ -ACGTACAGTCCGT -3’. What is the sequence of ′′the mRNA molecule?
  1. The following sequence is located in the middle of an mRNA molecule past the first start codon, so that it is written in the correct reading frame (the first three nucleotides are the first codon, etc.) 5’ – UGC CUG ACA UGC AUC ACG UGG G – 3’. Translate this into a polypeptide (write the amino acid sequence), starting at the beginning of this mRNA sequence (you don’t need to find a start codon in this case).
  1. Given a DNA template molecule of 5’ – TTATCTACCCTAATGGCCTAGACATATGG – 3’, generate the mRNA sequence and then the polypeptide sequence, starting at the first start codon.
  1. Given the DNA non-template molecule of 3’ – TACGGAATATAGTAAAACTAGTATATCG – 5’, generate the mRNA sequence and then the polypeptide sequence, starting at the first start codon.
  1. Given the tRNA anticodon of 3’ – ACG – 5’, what mRNA codon sequence would it bind during protein translation? What amino acid does this tRNA carry?

Homework Answers

Answer #1

1. 5’ -ACGTACAGTCCGT -3`

3`- TGCATGTCAGGCA 5`

m RNA-

5`ACGUACAGUCCGU 3`

2. 5’ – UGC CUG ACA UGC AUC ACG UGG G – 3’.

Protein-

N cys- leu- thr - cys - ile - thr - trp C

3.  5’ – TTATCTACCCTAATGGCCTAGACATATGG – 3`

3` - AATAGATGGGATTACCGGAYCTGTATACC 5`

m RNA-

5` UUAUCUACCCUA AUG GCC UAG ACAUAUGG 3`

Protein- N met- ala- stop C

4.  3’ – TAC GGA ATA TAG TAA AAC TAG TAT ATC G – 5’,

5` - AUG CCU UAU AUC AUU UUG AUC AUA UAG C 3`

Protein- N met- pro- tyr- ile- ile- leu - ile- ile- stop C

5. 3’ – ACG – 5’,

m RNA- 5` - UGC -3`

Amino acid- Cysteine

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
A DNA template has the following sequence: 5’-GCTTACTGGCCCGGTTTATTGCCCATCGCC-3’ Infer a transcribed mRNA sequence first, identify the...
A DNA template has the following sequence: 5’-GCTTACTGGCCCGGTTTATTGCCCATCGCC-3’ Infer a transcribed mRNA sequence first, identify the start codon, and determine the complete amino acid sequence that would be translated. Be specific about the amino-terminus and carboxyl-terminus.
10. Given the following DNA sequence as the template strand: TTACGATGCCCA Finish the complementary DNA strand....
10. Given the following DNA sequence as the template strand: TTACGATGCCCA Finish the complementary DNA strand. AAT _ _ _ _ _ _ _ _ _ Finish the corresponding mRNA (use template strand) AAU _ _ _ _ _ _ _ _ _ Finish the corresponding protein sequence from the mRNA. (Use the table on mRNA codons to identify the protein sequence. For example, the mRNA codon AAU codes for the amino acid, asparagine, Asn. Asn-____-____-____ What would be the...
A portion of a protein-coding gene is provided for this question. Given the non-template DNA sequence...
A portion of a protein-coding gene is provided for this question. Given the non-template DNA sequence of 5'-TACTATGCTGAGCATTATTGCTAAGTGATGGCTA-3': a. Write the complementary template DNA sequence in a 5' to 3' direction. b. Write the mRNA sequence that would be transcribed from this segment of DNA in a 5' to 3' direction. c. Starting from the first start codon, write the polypeptide sequence that would be translated from the mRNA sequence. d. Define the following terms in your own words: frameshift...
The following template sequence of DNA was isolated from a bacterial cell. Identify the promoter region...
The following template sequence of DNA was isolated from a bacterial cell. Identify the promoter region (assume there is no operator) and transcribe the DNA to the right of the promoter. Once you’ve transcribed the DNA into mRNA, find the start codon and translate the sequence into the correct amino acids to create a polypeptide. 3’-AATATTTATTATATAACCCGGCATACGTTCGGACGTCCCATATAGACTCCATTCGACTT-5’
4-3.  To demonstrate an understanding of translation elongation, we’ll start somewhere in the middle of translation of...
4-3.  To demonstrate an understanding of translation elongation, we’ll start somewhere in the middle of translation of a polypeptide.  At this moment, the entire ribosome (the small and large subunits) is bound to the mRNA, and there is a tRNA bound at the P-site.  Attached to the 3’-end of this tRNA is the start of the polypeptide- Amino-Met-Leu-Ser-Ala-Phe-tRNA At this starting point, there is no other tRNA bound to the ribosome, and the next codon is 5’-UGG-3’ When the next tRNA binds to...
Q2 An open reading frame (ORF) is a section of codons that begins with a start...
Q2 An open reading frame (ORF) is a section of codons that begins with a start codon and ends with a stop codon. Each codon specifies for a specific amino acid. ORF is the part of a gene that after transcription into an mRNA gets translated into a protein. With the help of several translation factors and enzymes, ribosomes translate mRNAs into proteins. The following DNA sequence contains a complete open reading frame (ORF) of a short transcriptional unit. The...
A codon is a series of three bases in mRNA which is equivalent to the 3...
A codon is a series of three bases in mRNA which is equivalent to the 3 bases in DNA; this triplet code corresponds to one amino acid. Once the mRNA is attached to a ribosome, it can assemble the protein by translating from the “language” of nucleic acids to the “language” of amino acids. What is the processed called? In order to synthesize a protein molecule, amino acids need to be aligned in the proper sequence along the RNA sequence....
*A DNA template sequence is 3’ ATG 5’. The complimentary mRNA codon sequence is: A 5’...
*A DNA template sequence is 3’ ATG 5’. The complimentary mRNA codon sequence is: A 5’ TAC 3’      B 5’ UAC 3’      C 3’ TAC 5’      D 3’ UAC 5’
A DNA template strand has the following base sequence: 5' TTACACGTGGACTGAGGACCTCTCCAT 3' 1.) What is sequence...
A DNA template strand has the following base sequence: 5' TTACACGTGGACTGAGGACCTCTCCAT 3' 1.) What is sequence of bases that in the complimentary strand of DNA? 2.) What is the mRNA that would be transcribed from this DNA?
Write the mRNA that would form for the following section of DNA. For each codon in...
Write the mRNA that would form for the following section of DNA. For each codon in the mRNA, write the amino acid that would be placed in the protein by a tRNA. 3.1. DNA strand: 3’—CCC—TCA—GGG—CGC—5’ mRNA: —______—______—______—______— amino acid order _______—_______—_______—_______ 3.2. DNA: 3’—ATA—GCC—TTT—GGC—AAC—5’ mRNA: —______—______—______—______—______— amino acid order —_______—_______—_______—_______—_______ • If a base substitution in DNA ( G replaces C in DNA ) ,changes the second codon in mRNA, how is the amino acid sequence affected in the...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT