Question

Calculate the hemoglobin concentration for the data sample if

Test Sample= 0.562

Cyanmethaemoglobin Standard = 0.4

Conc of Standard (g/L) = 0.527

the dilution factor is 1:250 (strictly 1 in 251)

Formula:

Hb conc.(g/100ml)= (test sample /cyanmethaemoglobin standard) x conc. standard (g/100ml) x Dilution factor

Please explain how to use the Dilution factor in the calculation.

Is it within the normal range?

How to calculate the mean if there are more similar data samples provided?

Answer 1

Hb conc.(g/100ml)= (test sample /cyanmethaemoglobin standard) x conc. standard (g/100ml) x Dilution factor

= 0.562/ 0.4    x  0.0527 g/100mL  x 251

= 18.58 g/100mL = 18.58 g/dL

Could be normal or higher range depending upon the age of individual.

The normal ranges are:

• Newborns: 17 to 22 g/dL
• One (1) week of age: 15 to 20 g/dL
• One (1) month of age: 11 to 15 g/dL
• Children: 11 to 13 g/dL
• Adult males: 14 to 18 g/dL
• Adult women: 12 to 16 g/dL
• Men after middle age: 12.4 to 14.9 g/dL
• Women after middle age: 11.7 to 13.8 g/dL

The mean is the average of the numbers. Add up all the numbers, then divide by how many numbers there are.