Question

What kinds of human DNA(which part)can do PCR? I need specific examples! Thank you.

Answer 1

PCR can be done all kinds of DNA. However, efficiency of PCR is highly compromised in case of template with high GC content (Guanine/cytosine content greater than 60%). High GC content region usually form secondary structures such as hairpins or loops. These structure hinders polymerase to perform its action. Also, denaturation is not easily achieved and annealing efficiencies differs. Usually these regions are found in promoters (located upstream) of various genes.

Thus, human DNA with low GC content (<50%) can do PCR at ease.

Examples are coding sequences usually structural part of genes.