Question

6. Table below shows the results from a transduction experiment. Calculate the number of transductants that has resulted from this experiment (show your calculations).

List 3 reasons for the bacterial growth on the control plates.

Culture	Transduction Minimal Medium
E.coli control 1	5
E.coli control 2	3
E.coli + P-22 plate 1	37
E.coli + P-22 plate 2	41

This is the whole question?  How do you calculate transductants from the experiment?? and What are 3 reasons bacterial grows on control plates?

Answer 1

Transductant is a bacterial cell into which material has been transduced or a cell or organism (bacterium) that has undergone transduction.

The probability of successful infection with lambda viruses per cell is 1-(1-r) lambda and the total number of infected cell is I=T. The transduction efficiency (TE) is the percentage of successful infected cell in the total cells, so TE= I/T= 1-(1-r) lambda.

Bacteria grow best at 37°C which is body temperature. Bacteria like moist conditions. Bacteria grow best in a neutral pH between 6.6 and 7.5. Most bacteria needs oxygen to survive. Warm and dark environment is needed.