Question

The following is a list of four main pigments found in plants in order of polarity:...

The following is a list of four main pigments found in plants in order of polarity: (Most Polar- Least Polar) Chlorophyll b, Chlorophyll a, Xanthophylls, Carotenes. In a polar solution, which pigment would be closest to the solvent front? What would happen in a non-polar solution? Please explain

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Answer #1

The following is a list of four main pigments found in plants in order of polarity: (Most Polar- Least Polar) Chlorophyll b, Chlorophyll a, Xanthophylls, Carotenes. In a polar solution, which pigment would be closest to the solvent front? What would happen in a non-polar solution? Please explain

From most polar pigment to leat polar segment in the order of Chlorophyll b, Chlorophyll a, Xanthophylls, Carotenes. The pigment which would be closest to the solvent front is the carotens because it is least polar pigment.

Chlorophyll b, Chlorophyll a, Xanthophylls, Carotenes are photosynthetic pigments of palnts located on the leaf's chloroplast. The experiment by which these pigments are seperated is thin-layer chromatography based on their affinities for the stationary phase that is the polar molecule and the mobile phase consist of solvent non-polar molecule.

In TLC, high solvent affinity compounds will move much further than high polar affinity molecules. In this case, carotenes are least polar pigment with high solvent affinity will move towards the solvent front and far away than the chlorophyll b which is more polar molecules.

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