Question

You are required to make a 10ml PBS (Phosphate-buffered saline) sodium phosphate buffer. 7.5pH, 200mM how...

You are required to make a 10ml PBS (Phosphate-buffered saline) sodium phosphate buffer. 7.5pH, 200mM

how many grams of acid and how many grams of base do we need? how much demi water is required?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Sodium Phosphate Buffer is a mixing of Sodium Dihydrogen phosphate NaH2PO4H2O ( Acid) and Disodium Hydrogen Phosphate Na2HPO4-7H2O (Base).

we have to prepare 10 ml of Sodium Phosphate Buffer with 7.5 pH.

  • Add 8 ml distilled water in a container.
  • Add 0.202 g of Na2HPO4-7H2O to the solution.
  • Add 0.034 g of NaH2PO4H2O to the solution.
  • Adjust the solution to get final pH by using Hcl or NaoH.
  • Add distilled water upto volume is 0.01 L.

And finally we obtain 10 ml of Sodium Phosphate Buffer solution.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
You're asked to prepare 10mL of a PBS solution (phosphate buffer saline) containing 40% glycerol. All...
You're asked to prepare 10mL of a PBS solution (phosphate buffer saline) containing 40% glycerol. All you have is a 10X PBS solution (10 times more concentrated than the solution you're asked to prepare). How are you going to procede in order to prepare a 1X PBS solution containing 40% glycerol? b) What is the molarity of the glycerol in solution? (given: Density of glycerol is 1.257g/cm^3 and molar mass is 92.1g/mol)
Phosphate-buffered saline (PBS) is one of the commonly used buffers to maintain the pH value during...
Phosphate-buffered saline (PBS) is one of the commonly used buffers to maintain the pH value during the purification of biology related samples. Below are all the components for PBS buffer: NaCl, KCl, Na2HPO4 and KH2PO4. A. Please identify the acidic and basic component of the buffer. (Hint: acidic and basic components should be a conjugate acid-base pair.) B. Please write the chemical equation that will happen when additional HCl is added into the buffer. (Hint: acids like to react with...
You need to prepare a buffer for biochemistry lab. The required solution is 0.5Msodium phosphate, pH...
You need to prepare a buffer for biochemistry lab. The required solution is 0.5Msodium phosphate, pH 7.0. Use the Henderson-Hasselbalch equation to calculate the number of moles and grams of moobasic sodium phosphate ( NaH2PO4) and dibasic sodium phosphate ( Na2HPO4) necessary to make 1 liter of solution. The pKa for this buffer is 7.21
how many grams of sodium phosphate would you need to make 275 ml of 7.75 m...
how many grams of sodium phosphate would you need to make 275 ml of 7.75 m stock solution?
Phosphoric acid (H3PO4) is a triprotic acid with the following pKa values: pKa1 = 2.15 pKa2...
Phosphoric acid (H3PO4) is a triprotic acid with the following pKa values: pKa1 = 2.15 pKa2 = 7.20 pKa3 = 12.35 a). You wish to prepare a 1.000 L of a 0.0450 M phosphate buffer at pH 6.8. Which of the following salts do you use (PICK 2) and what mass of each salt will you add to the mixture? H3PO4 (M.W.= 97.995) KH2PO4 (M.W.=136.086) Na2HPO4 (M.W.=141.959) AlPO4 (M.W.=121.953) b). A concentrated phosphate buffer known as “phosphate buffered saline” (or...
You want to make 100 mL of 0.20 M Acetic Acid buffer with pH=4.0 You are...
You want to make 100 mL of 0.20 M Acetic Acid buffer with pH=4.0 You are given a stock solution of 1.0 M acetic acid and a bottle of sodium acetate salt (MW= 82 g/mol). The formula for the dissociation of acetic acid is shown here (CH3COOH <--> CH3COO- + H+) The henderson hasselbach equation is : pH=pKa +log [A-]/[HA]. What is the ratio of [A-]/[HA] when your buffer pH is 4.0? Determine the concentration of weak acid and conjugate...
A buffer made of 250 mM sucrose and 50 mM sodium phosphate is used to homogenize...
A buffer made of 250 mM sucrose and 50 mM sodium phosphate is used to homogenize the tissues for this experiment. You have 2M sodium phosphate buffer and dry sucrose. How would you make 750 ml of the buffer? Show your calculations. (HINT: there is one piece of information that is NOT given that you need to find out in order to answer this)
Which acid would you choose to combine with its sodium salt to make a solution buffered...
Which acid would you choose to combine with its sodium salt to make a solution buffered at pH 4.2 For the best choice, calculate the ratio of the conjugate base to the acid required to attain the desired pH. Chlorous Acid (HClO2) pKa=1.95 Nitrous Acid (HNO2) pKa=3.34 Formic Acid (HCHO2) pKa=3.74 Hypochlorous Acid (HClO) pKa=7.54
if you were asked to make up a pH=6.86, 0.1M phosphate buffer, how would you do...
if you were asked to make up a pH=6.86, 0.1M phosphate buffer, how would you do this? Using the henderson-hasselbach equation, plug in the pH and the Pka for sodium phosphate monobasic (6.86) for this situtation, what is the log(A-/HA)=
a) Which of the two of the following compounds would you mix to make a buffer...
a) Which of the two of the following compounds would you mix to make a buffer of pH 7.45 phosphoric acid (FM=98.00), sodium dihydrogen phosphate (FM=119.98), disodium hydrogen phosphate (FM=141.96), or sodium phosphate (FM=163.94)? b) If you wanted to prepare 1.pp L of buffer with a total phosphate concentration of 0.0500M, how man grams of the selected compounds would you mix together? c) If you did what you calculated in part (b), you would not get a pH of exactly...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT