If a strand of DNA of sequence 5’-AATAGCGCGGTATTC-3’ is replicated, which of the following accurately represents the newly synthesized DNA strand?
Ans.
The answer is 3’-TTATCGCGCCATAAG-5’
Explanation:
In a double stranded DNA, the DNA strands are so arranged that their nucelotides face each other and both are held together by a hydrogen bond.This face to face arrangement of the nucleotides results in an opposite orientation of the two strands.That is, when one of the DNA strand is 5' to 3' direction, the other strand will be oppositely oriented in a 3' to 5' direction. Also, in a DNA strand the nucleotide bases are complementary to each other and we know that according the complementary base pairing rule or the Chargaff's rule, Adenine (A) pairs with thymine (T) and guanine (G) pairs with cytosine (C) always.
Hence for the given DNA sequence 5’-AATAGCGCGGTATTC-3’, the newly synthesized DNA strand will be
3’-TTATCGCGCCATAAG-5’
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