Question

This protein initially activates siRNA? None of the answers are correct DICER Target mRNA RISC

This protein initially activates siRNA?

None of the answers are correct

DICER

Target mRNA

RISC

Homework Answers

Answer #1

Ans.

  • Small interfering RNA, or as also called, short interfering RNA/silencing RNA (siRNA), belongs to a group of double-stranded RNA non-coding RNA molecules.
  • It is like miRNA and is a part of the RNA interference (RNAi) pathway.
  • It intervens with specific genes expression containing complementary sequence of nucleotide through mRNA degredation post transcription.
  • By degrading mRNA it inhibits the translation of proteins.
  • Structure of siRNA is well defined containing a short double stranded RNA having phosphorylated 5' ends and a 3' hydroxlated end.
  • The RISC protein function to initially activates the siRNA and activity of siRNA in RNAi is dependent largely on the binding efficiency of the siRNA to RNA-induced silencing complex (RISC).
  • Duplex siRNA binding to RISC results in unwinding and cleavage of sense strands by endonucleases.
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