Question

Sample of diarrhea -  Esherichia coli

            Which plates are you going to use to culture the material?  Choose two and explain use

            What is the ingredient in the selective plate?  What is the ingredient in the differential plate?  What would you see if this plate has growing on it?  What antibiotic would you suggest (Use the figure from Ch12 in the Cowan book)

Answer 1

A) - Lauria-bertani media contain tryptophan and yeast extract

Mac conkey agar- contain lactose. Both of these agar helps in growth of ecoli and differentiating from other intestinal flora

B)- selective plate is of macconkey agar which contain- peptone , lactose , agar , neutral red,N aCl .

C) - MUG Lauryl sulphate broth- composition - casein enzyme hydrosylate,lactose,NaCl,m onopotassium phosphate , dipotassium phosphate

D)-luxuriant , red ring at the interface of the medium

E)- chloramphenicol

Ciprofloxacin

Gentamicin

Norfloxacin