Question

The yeast GAL1, GAL7, and GAL10 genes encode enzymes necessary to metabolize the sugar galactose. Will...

The yeast GAL1, GAL7, and GAL10 genes encode enzymes necessary to metabolize the sugar galactose. Will these structural genes be expressed in diploid yeast with the following genotypes and under the given conditions?

GAL80 signifies the wild type allele, while gal80- signifies a loss of function mutation.

GAL4 signifies the wild type allele and gal4- signifies a loss of function mutation

gal4D encodes a protein will not interact with GAL80 protein.

gal3- is a loss of function mutation in GAL3.

If a gene is not listed, assume it is wild type.

Glucose is NOT in either medium.                                                                                                                            

Genotype

Medium without galactose

Medium with galactose

GAL80 GAL4 / GAL80 GAL4

gal80- GAL4 / gal 80- GAL4

GAL80 gal4- / GAL80 GAL4

GAL80 gal4- / GAL80 gal4-

GAL80 gal4D / gal80- GAL4

gal80- gal4- / gal80- gal4-

gal3- GAL4 / gal3- gal4-

gal3- gal80- / gal3- gal80-
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Answer #1
  

Genotype

 Medium without galactose Medium with galactose
GAL80 GAL4 / GAL80 GAL4 NO EXPRESSION EXPRESSION OCCURS
gal80- GAL4 / gal 80- GAL4 EXPRESSION OCCURS EXPRESSION OCCURS
GAL80 gal4- / GAL80 GAL4 NO EXPRESSION EXPRESSION OCCURS
GAL80 gal4- / GAL80 gal4- NO EXPRESSION NO EXPRESSION
GAL80 gal4D / gal80- GAL4 EXPRESSION OCCURS EXPRESSION OCCURS

gal80- gal4- / gal80- gal4-

NO EXPRESSION

NO EXPRESSION

gal3- GAL4 / gal3- gal4-

EXPRESSION OCCURS

EXPRESSION OCCURS

gal3- gal80- / gal3- gal80-

 EXPRESSION OCCURS EXPRESSION OCCURS
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