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Which of the following reactions with covalently closed, circular double stranded DNA (ccc dsDNA) does NOT...

Which of the following reactions with covalently closed, circular double stranded DNA (ccc dsDNA) does NOT result in a new linking number? Select one choice from the list below (A-D) and explain your answer.

A. incubation with a type I topoisomerase

B. incubation with a type II topoisomerase

C. incubation with ethidium bromide

D. a brief incubation with DNase I followed by resealing the breaks in the phosphodiester backbone with another enzyme (DNA ligase)

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Answer #1

In cases A,B and D linking number is changed but in case C linking number remain the same.

So correct answer is option"C"

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