Question

Lac Operon

1. Drawing and mapping the lac operon genes and discussing in pros=words

2. What types of mutations affect that she has made and how it will affect the lac operon?

3. ITPG what is it used for to manipulate the lac Operon

4. Faith of viruses and different classes

5. Regulation of operons negative or positive repression or induction

6. Kreb Cycle/ ETC

Answer 1

1)The CAP-cAMP complex binds to the lac promoter, Mutants whose lac gene is not stimulated by complex had the mutation in the lac promoter. Mapping the DNA has shown that the activatorbinding site lies just upstream of the promoter. Binding of CAP and cAMP to the activator site helps RNA polymerase form an open promoter complex.

2) Mutations in lacl cause constitutive expression of beta galactosidase and lactose premase beacause no functional repressor is made

3) Used as an inducer