Question

Which one of the following temperatures would most likely kill a mesophile (numbers expressed in degrees Celsius)?

60

37

0

9

-50

Which is an indirect method of measuring microbial growth?

		MPN (Most Probabable Number)
		filtration
		microscopic count
		serial dilutions
		metabolic activity

Answer 1

Ans 60 0C

because 37 is optimum and lower temperature will lead to protein crystalization but some cells might be able to survive but higher temperature eliminate mesophiles at 60 celsius. A mesophile is an organism that grows best in moderate temperature, neither too hot nor too cold, typically between 20 and 45 °C.

Ans Metabolic activity

An indirect way of estimating bacterial numbers is measuring the metabolic activity of the population (for example, acid production or oxygen consumption). For filamentous organisms such as fungi and molds, measuring dry weight is a convenient method of growth measurement.