The absorbance of a chemical solution is 0.5 at 400 nm. The concentration of the solution is 50 mM. The pathlength of the cuvette is 1 cm. What is the molar absorptivity of the chemical at this wavelength?
The formula for absorbance is :- A = ɛ x l x c
In this, A is absorbance, ɛ is molar abaorptiviy, l is length of the cuvette and c is the concentration of the solution.
Now we can move the equation for ɛ and it becomes :-
ɛ = A/lc (Here we took lc to the other side)
Now we have the values of A, l and c given.
A is 0.5
c is 50mM = 0.05 M (We converted it into M by dividing 50 with 1000)
l is 1 cm
So putting the values in the equation
ɛ = 0.5/(1 X 0.05)
ɛ = 10 L Mol-1 cm-1
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