Question

D._____ Examine the structure of a fruit fly wing blade (which is flat) to determine how many veins it contains and their pattern

1. Atomic force microscopy (AFM)

2. Light microscopy, standard

3. Light microscopy with differential interference contrast (DIC)

4. Scanning electron microscopy (SEM)

5. Transmission electron microscopy (TEM)

6. Fluorescence microscopy, standard

7. Laser scanning confocal microscopy (LSCM)

8. Live cell imaging of GFP-tagged protein

9. Fluorescence resonance energy transfer (FRET)

Answer 1

D. Atomic force microscopy is a type of scanning probe microscopy that generate image when a centilever move over the surface of sample. The pattern of fly wing obtained with highly stereotyped blade structure having five longitudinal and two transverse veins. Thus,  arrangement of veins seems to be stereotyped, providing an intriguing puzzle in developmental patterning. On both the surface of adult wings, vein cells are recognized having thick cuticle and are more densely packed than the intervein cells found between veins.