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What organism do the biological indicator vials contain? (Be specific!) What structure does this organism form...

What organism do the biological indicator vials contain? (Be specific!)

What structure does this organism form that makes it useful as a biological indicator for autoclaving?

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Answer #1

The bacteria specifically used as biological indicator is Geobacillus stearothermophilus.

This bacteria form spores which are activated when autoclaved at 121.1oC in saturated steam for a minimum duration of 20 minutes. The bacterium comes in spore form on strips or as a suspension in prepackaged vials.  These are given in vials during autoclave> after autoclaving, these are incubated in growth medium under suitable condition. If no growth of the bacteria occur, then we can conclude that the autoclave is perfect. Thus we can check the quality of sterilization of the vials which are autoclaved..

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