Question

1. Transcribe and translate the normal sequence and the mutated sequence.
2. Identify what type of DNA mutation occurred. (insertion, deletion, or substitution)
3. Identify what type of protein change resulted. (frameshift, missense, nonsense, or silent)

Normal Sequence 3’ TACCTTCACCGCAGGGTAGCTTTTATTTAA 5’

Mutated sequence 3’ TACCTTCACCGCAGGTAGCTTTTATTTAA 5’

Answer 1

Ans a)

Normal sequence-

3’ TACCTTCACCGCAGGGTAGCTTTTATTTAA 5'

After transcription :-

3' AUG GAA GUG GCG UCC CAU CGA AAA UAA AUU - 5'

After translation

Protein - Met- glu-val-ala-ser-his-arg-asn stop codon

Mutated sequence -

3’ TACCTTCACCGCAGGTAGCTTTTATTTAA 5

After transcription :-

3' AUG GAA GUG GCG UCC AUC GAA AAU AAA UU 5'

After translation -

Met- glu-val-ala-ser-ile-glu-asn-lys

b) it is deletion, since G is deleted at the 14th position.

c) it is frame shift mutation, since it lead to change in the open reading frame and thus change the type of protein formed.