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Why must IPTG/X-gal/Ampicillin plates be incubated no longer than 18 hours? Plates were placed at 37*C...

Why must IPTG/X-gal/Ampicillin plates be incubated no longer than 18 hours?

Plates were placed at 37*C for 12-18 hours

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Answer #1

I believe the correct answer to be:

This is because they are all the compounds that will be degraded after a long time such as ampicillin which has a life and once the concentration of such compound falls below a point, a normal cell would be able to grow which was inhibited by ampicillin before which mau interfere with the result and may cause contamination.

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