Question

You need to make serial dilution of a cell suspension and you start at 1:10 dilution...

You need to make serial dilution of a cell suspension and you start at 1:10 dilution and need to go all the way to 10-6 dilution. How many test tubes you need to make up all your dilutions?

Homework Answers

Answer #1

Answer - 6 test tube for all the dilution

Reason

Forthe serial dilution of a cell suspension if you start with the 1:10 dilution then that test tube is called as 10-1 , the next test tube will have dilution 1: 100 then the next test tube will be called as 10-2 . If we continue this till 5th test tube the dilution will be 1:100000 and the it will be called as 10-6. The total test test tubes required to make all dilution is 6. The first testube will have 1:10 dilution and the 6th tube will have 1:1000000 dilution i.e 10-6.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
You will prepare a serial dilution with 6 tubes. Into tube 1 you add 0.9 ml...
You will prepare a serial dilution with 6 tubes. Into tube 1 you add 0.9 ml NaCl and you add 0.5 ml NaCl into tubes 2-6. Next you add 0.1 ml of serum to tube 1 and mix and transfer 0.5 ml to tube 2. Continue making serial dilutions through tube 6 (discarding 0.5 ml from tube 6). Finally, you add 0.5 ml of sheep RBCs to EACH tube (1-6) What is the final dilution in each tube after the...
You have a cell suspension that has 107 cells/mL and you serially dilute the culture to...
You have a cell suspension that has 107 cells/mL and you serially dilute the culture to obtain the following dilutions: 10-1, 10-2, 10-3, 10-4, 10-5, 10-6. Which of the dilutions will yield a “countable” number of colonies (30-300 per plate) when you plate 0.1 ml of each dilution? Show your work.
Q1. You need to make 10 ml of a restriction enzyme buffer. You have the the...
Q1. You need to make 10 ml of a restriction enzyme buffer. You have the the following stock solutions: 50 mM Tris-HCl, 0.10 M MgCl2, 50. mM DTT, distilled water. How much of each ingredient and water do you need to make the buffer to these final concentrations of ingredients: ·            6.0 mM Tris-HCl ·            8.0 mM MgCl2 ·            1.0 mM DTT. How much water (in ml) do you need? Q2. Derrick later gets a laugh on Jenny. Jenny’s boss, Repunzelee, asks her...
Serial dilutions Please draw how to prepare this serial dilution prepare a series of p nitrophenyl...
Serial dilutions Please draw how to prepare this serial dilution prepare a series of p nitrophenyl phosphate concentrations from 1x10^2 M from to 3.125x10^-4 using the DEA buffer as diluent. do this by srial diluting the 1x10^-2M stock by 1/2 so you end up with 2 ml each of p-nitrophenyl phosphate concentrations of 5x10^-3M, 2.5X10^-3M, 1.25x10^-3M, 6.25x10^-4M and 3.125x10^-4M
Using a 1:1 dilution scheme, how many dilutions would you need to do to prepare a...
Using a 1:1 dilution scheme, how many dilutions would you need to do to prepare a 62.5 mM solution from a stock solution of 1 M?
Total Heterotrophic Plate Count: A serial dilution was conducted using standard methods, then selected dilutions were...
Total Heterotrophic Plate Count: A serial dilution was conducted using standard methods, then selected dilutions were used to set up pour plates with total plate count agar, and plates incubated for 48 – 72 hours. Each dilution was plated in duplicate, with 1.0 ml of inoculum placed into Petri plates, then pour plates prepared. After incubation, you counted the following: 10*-3 dilution: Too many to count on both plates. 10*-4 dilution: 378 colonies; 427 colonies. 10*-5 dilution: 53 colonies; 47...
Illustrate how you would make four serial 3-fold dilution such that each tube has a volume...
Illustrate how you would make four serial 3-fold dilution such that each tube has a volume of 12mL. There shouldbe at least 6 mL in each tube following the preparation of all diluions because that is a minimal requirement for this particular experimental setup?
Explain how to: -Make 1/100 dilution of the yeast suspension using normal saline as diluent. -Mix...
Explain how to: -Make 1/100 dilution of the yeast suspension using normal saline as diluent. -Mix equal volumes of yeast and Trypan blue -Load the hemocytometer. If your sample is too dense, dilute your sample some more
You are given a culture of bacterial cells of unknown concentration. You are asked to determine...
You are given a culture of bacterial cells of unknown concentration. You are asked to determine the concentration (cells/mL). You make three serial dilutions of 100-fold and a final 10-fold dilution of the sample. After plating 1 mL of this culture dilution on a growth plate, you generate 42 bacterial colonies (each colony represents a single bacterial cell that was on the plate). What was the concentration of the original bacterial cell culture?
6. (16 pts.) When you performed (or will perform) the atomic absorption experiment, you performed parallel...
6. (16 pts.) When you performed (or will perform) the atomic absorption experiment, you performed parallel dilutions (in lieu of serial dilutions) with a Zn2+ standardized solution, and used your analysis of these dilutions to construct a calibration curve relating the sensitivity of the instrument to the Zn2+ concentration In this problem, you are going to quantify the uncertainty in the concentrations of Zn2+ dilutions obtained from both serial and parallel dilutions and compare the results for the two methods....