Question

in molecular taxonomy,what is ITS region and why is it so useful?

in molecular taxonomy,what is ITS region and why is it so useful?

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Answer #1

ITS region or Internal Transcribed Spacer region is a non-functional spacer DNA that is situated between two genes ( situated between structural ribosomal RNA (rRNA) on a precursor transcript.)

Uses of ITS region

  • Known as the Universal Fungal Barcode because ITS region is the widely sequenced region in fungi for identifying molecular ecology.
  • Used to identify the geographical races of species and also used to identify the molecular systematics of the species in its genus levels.
  • Used to resolve the species and isolates species in the same genus.
  • ITS region variability differs from taxa to taxa.
  • ITS region is same in same genus and is used as a similarity cutoff for identifying the same genus species.
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