Question

what sequencing method should you use to sequence a cancer patient's exome ?Explain why and give...

what sequencing method should you use to sequence a cancer patient's exome ?Explain why and give a summary of the approach.

Homework Answers

Answer #1

Parallel sequencing, providing a faster turnaround time than Sanger sequencing or shotgunsequencing. Exome sequencingpanels can be expanded to include noncoding genes and other genetic elements that are relevant to cancer.

parallel sequencingexperiments can be used to detect chromosomal copy number variants (CNVs), translocations, and other structural variations .Methodsto detect somatic CNVs in cancerover come this issue by directly comparing read depths between a tumor and matched normal.

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