Question

What is the mechanism of action of Chloramphenicol? A. Blocks the “P” site B. Blocks peptidyl...

What is the mechanism of action of Chloramphenicol?
A.
Blocks the “P” site
B.
Blocks peptidyl transferase of bacteria
C.
Blocks the peptidyl transferase of 80S eukaryotic ribosome
D.
Blocks the “A” site
E.
Misreading of the genetic frames
2.
What is the mechanism of action of Cycloheximide?
A.
Blocks peptidyl transferase of bacteria
B.
Misreading of the genetic frames
C.
Blocks the peptidyl transferase of 80S eukaryotic ribosome
D.
Blocks the “A” site
E.
Blocks the “P” site
3. What is the mechanism of action of Puromycin?
A.
Blocks peptidyl transferase of bacteria
B.
Blocks the “P” site
C.
Misreading of the genetic frames
D.
Blocks the “A” site
E.
Blocks the peptidyl transferase of 80S eukaryotic ribosome
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Answer #1

Answer - Mechanism of action of Chloramphenicol

Option B - Blocks the peptidyl transferase of bacteria.

Chloramphenicol is a bacteriostatic drug which inhibits protein synthesis in bacteria by inhibiting peptidyl transferase activity of bacterial ribosome.

Answer - Mechanism of action of Cycloheximide

Option C - Blocks the peptidyl transferase of 80S eukaryotic ribosome

Cycloheximide is a protein synthesis inhibitor in Eukaryotes whose mechanism of action is similar to Chloramphenicol. It inhibits translation elongation by binding to E site of ribosome.

Answer - Mechanism of action of Puromycin

Option B - Blocks the “P” site

Puromycin is a ribosome-targeting antibiotic that quantitatively deacylates the P-site peptidyl-tRNA. It acts on Eukaryotes as well as prokaryotes.

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