Question

How would you conduct an experiment using Chip-Seq technique and specific antibodies against methylated DNA, to find imprinted genes in the human genome? (Hint: start with making separate genomic DNA preparations from male and female cells).

Answer 1

Chip sequence technique also known as micro array sequencing or comparative hybridization ( compare genome after hybridization)

Can detect unknown Gene locus

interpretation-

red -gain of Gene eg amplification or mutation

Yellow-Normal areas

Green-loss of Gene eg deletion