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1. During the reverse transcription reaction, what occurs at each of the reaction incubation times (5...

1. During the reverse transcription reaction, what occurs at each of the reaction incubation times (5 min at 25º C; 30 min at 42 º C: and 5 min at 85 ºC)?

2. What are the differences between qPCR and end-point PCR?

3. What are the advantages of carrying out qPCR versus endpoint PCR?

4. Why is the internal control used in qPCR?

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