Question

How could you use ATAC-seq to treat human progeria?

How might you use 5-Azacytidine(AZA) to treat progressive human blindness?

How might you use AZA to treat human progeria?

How could you use DNA editing of secondary splicing sequences to correct progeria?

How could you test for changes in mitochondrial function occurring during spaceflight? What would you expect the results to show?

How might you design a life-extension therapy for progeria patients?

Explain how ChIP-qPCR could be used to find and describe genes that allow species to thrive in weightless(space) conditions.

human progeria

Answer 1

ATAC seq identifies the accessible DNA regions by providing open chromatin with hyperactive mutant Tn5 Transposase that inserts sequencing adapters into open regions of genome. The tagged DNA fragments are purified, PCR amplified and then sequenced using next generation sequencing. This is how ATAC seq treats human progeria.

When AZA is used,it undergoes demethylation or interferes with the methylation of DNA. In the process, tumor suppressor genes are restored, thus helping in treating blindness.