PLOT DATA?
In this experiment, each student received 5 tubes of each of the following: Broth A (no sugar, no buffer), Broth B (1.0% glucose, no buffer), Broth C (1.0% glucose, buffer, and Broth D (0.1% glucose, buffer). Five organisms, which included 3 bacterial cultures, one yeast, and one mold as indicated in the table below, were used to inoculate the broths. The tubes were then incubated for 24 hours at 37°C. On day 2, the pH of each tube was approximated using a separate strip of pH paper and recoded. Data from all students was pooled and averaged. The turbidity of each of the broths was measured spectrophotometrically using a wavelength of 650 nm. Data from all students was again pooled and averaged.
Pooled Results for Broth pH and Broth Absorbance |
|||
Broth Type |
Organism |
Average pH |
Average Abs. |
A |
Staph. epi |
8.0 |
0.594 |
E. coli |
8.0 |
0.692 |
|
S. cereviseae |
6.0 |
0.146 |
|
Penicillium |
8.0 |
0.022 |
|
Ent. faecalis |
6.0 |
0.053 |
|
B |
Staph. epi |
5.5 |
0.592 |
E. coli |
6.0 |
1.120 |
|
S. cereviseae |
6.5 |
0.041 |
|
Penicillium |
7.5 |
0.023 |
|
Ent. faecalis |
5.0 |
0.444 |
|
C |
Staph. epi |
5.5 |
0.596 |
E. coli |
6.0 |
1.038 |
|
S. cerevisiae |
6.5 |
0.128 |
|
Penicillium |
7.5 |
0.093 |
|
Ent. faecalis |
5.0 |
0.789 |
D |
Staph. epi |
8.0 |
1.222 |
E. coli |
8.0 |
0.748 |
|
S. cereviseae |
7.5 |
0.093 |
|
Penicillium |
8.0 |
0.079 |
|
Ent. faecalis |
7.0 |
0.0257 |
this is the required graph
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