Question

PLOT DATA? In this experiment, each student received 5 tubes of each of the following: Broth...

PLOT DATA?

In this experiment, each student received 5 tubes of each of the following: Broth A (no sugar, no buffer), Broth B (1.0% glucose, no buffer), Broth C (1.0% glucose, buffer, and Broth D (0.1% glucose, buffer). Five organisms, which included 3 bacterial cultures, one yeast, and one mold as indicated in the table below, were used to inoculate the broths. The tubes were then incubated for 24 hours at 37°C. On day 2, the pH of each tube was approximated using a separate strip of pH paper and recoded. Data from all students was pooled and averaged. The turbidity of each of the broths was measured spectrophotometrically using a wavelength of 650 nm. Data from all students was again pooled and averaged.

Pooled Results for Broth pH and Broth Absorbance

Broth Type

Organism

Average pH

Average Abs.

A

Staph. epi

8.0

0.594

E. coli

8.0

0.692

S. cereviseae

6.0

0.146

Penicillium

8.0

0.022

Ent. faecalis

6.0

0.053

B

Staph. epi

5.5

0.592

E. coli

6.0

1.120

S. cereviseae

6.5

0.041

Penicillium

7.5

0.023

Ent. faecalis

5.0

0.444

C

Staph. epi

5.5

0.596

E. coli

6.0

1.038

S. cerevisiae

6.5

0.128

Penicillium

7.5

0.093

Ent. faecalis

5.0

0.789

D

Staph. epi

8.0

1.222

E. coli

8.0

0.748

S. cereviseae

7.5

0.093

Penicillium

8.0

0.079

Ent. faecalis

7.0

0.0257

Homework Answers

Answer #1

this is the required graph

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