Question

What is the role of p-Nitrophenol in enzyme assay and how do you measure it?

Answer 1

Ans) P nitrophenol is a substrate which is non-specific and non proteinaceous generally used for the assay of acid and alkaline phophatases. The Advantage of using P nitrophenol assay, is that the concentration of the substrate is higher than Michaelis constant(Km).

Enzyme activity will calculate that in how much quantity is the active enzyme present and is highly dependent on the specific conditions like enzyme concentration, temperature, substrate concentration, and pH concentration
Enzyme activity = rate x reaction volume.
Also, it can be calculated as :
Product concentration per unit time .
Where time is taken in minutes.

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