Question

Question 2(BOTANY)

How will you ensure that the PCR amplicon that you have obtained is the correct gene from devil’s claw? Describe the steps that you will follow in detail and provide all the details of the techniques that you use in each step.      

Answer 1

After gene amplification we can check at different stages-

1) expected size of gene should match with actual size of gene while in gel electrophoresis

2) once pcr is done we can go for gene sequencing and then analyses after gene sequencing ncbi blast

3) once everything is pefect then we go for cloning and then expression of protein will be checked with size of protein in terms of mol wt.

4) protein activity is the final checking point which should show actual expected result.

Thanks..