Question

How to make 50 mM of cisplatin (MW: 300 g/mol) in 100 microliter of DMSO. Show your work.

Answer 1

volume of the solution = 100 uL

1 L= 10^6 uL

1 uL= 10^-6 L

100 uL= 100 10^-6 L= 10^-4 L

molarity= number of moles/volume of the solution litres.

1 M= 10^3 mM

50 mM= 50 10^-3 M

50 10^-3 M = number of moles/ 10^-4 L

number of moles= 50 10^-3 10^-4

= 50 10^-7

= 5 10^-6

number of moles= mass in gram/gram molecular mass

5 10^-6 = mass in gram/300

mass in gram= 5 10^-6 300

= 1.5 10^-3 g

take 1.5 10^-3 g of cisplatin and dissolve it in DMSO such that the final volume= 100 uL