Question

What did Okazaki and Okazaki (1968) find when they compared the sizes of new DNA fragments...

What did Okazaki and Okazaki (1968) find when they compared the sizes of new DNA fragments that were in the process of being synthesized after stopping replication prematurely at different time intervals?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Okazaki fragments are short sequences of DNA nucleotides which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication. Because the lagging strand needs to be replicated in the opposite direction of the way the replication fork is proceeding. And DNA polymerase can proceed only 5' to 3'. Thus, short fragments are produced as the replication fork expands. The length of Okazaki fragments in the lagging strand is about 100-200 nucleotides in eukaryotes and about 1000-2000 nucleotides in prokaryotes.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Question 3 (Adapted from Okazaki, R., et al. 1968. Proc Natl Acad Sci USA 59:598–605) After...
Question 3 (Adapted from Okazaki, R., et al. 1968. Proc Natl Acad Sci USA 59:598–605) After the discovery of DNA polymerase by Arthur Kornberg, the properties of the enzyme became quite well known. One of the most critical is that all known DNA polymerases synthesize DNA only: 5' to 3'. This fact led to a dilemma regarding how the semiconservative model would work for a DNA molecule. Reiji Okazaki a brilliant experimenter took on this problem. [An aside: Okazaki was...
In the experiment, Okazaki cultured E. coli (at low temperatues to slow down replication process) in...
In the experiment, Okazaki cultured E. coli (at low temperatues to slow down replication process) in the presence of radioactive nucleotides. They did this for short pulses followed by the addition of excess nonradioactive nucleotides. This resulted in label (radioactivity) being present only in the DNA that was synthesized during the short period of the pulse. Soon after the pulse, they isolated the DNA and separated the individual strands from one another. The various pieces of DNA could then be...
1.What is the role of telomerase in DNA replication? a) join Okazaki fragments together b) synthesize...
1.What is the role of telomerase in DNA replication? a) join Okazaki fragments together b) synthesize RNA nucleotides to make a primer c) catalyze the lengthening of the ends of linear chromosomes d) are part of the initiation of replication at the origin of replication 2. RNA polymerase for mRNA transcription and DNA polymerase in replication differ in that a) RNA polymerase uses RNA as a template, and DNA polymerase uses a DNA template. b) RNA polymerase does not need...
7. DNA synthesis involves the polymerization of nucleotides into polynucleotide chains. In this concern answer the...
7. DNA synthesis involves the polymerization of nucleotides into polynucleotide chains. In this concern answer the following questions      a. Define and indicate the significance of ; i. Okazaki fragments, ii. DNA ligase, and iii. RNA primer during DNA replication. b. Several temperature-sensitive mutant strains of E. coli display the following characteristics. Predict what enzyme or function is being affected by each mutation. i. Newly synthesized DNA contains many mismatched base pairs. ii. Okazaki fragments accumulate, and DNA synthesis is...
Construct the physical map of a DNA molecule from which fragments of different sizes were generated...
Construct the physical map of a DNA molecule from which fragments of different sizes were generated after digestion with the following restriction enzymes: -ApaI: generates a 5.8 kb fragment -BamHI: generates two fragments of 2.5 and 3.3 kb -PstI: generates a 5.8 kb snippet -ApaI / BamHI: generate three fragments of 1.3, 2.0 and 2.5 kb -ApaI / PstI: generate two fragments of 1.0 and 4.8 kb -BamHI / PstI: generate three fragments of 0.3, 2.5 and 3.0 kb -ApaI...
Which of the following statements about DNA repair is correct during excision repair, an exonuclease is...
Which of the following statements about DNA repair is correct during excision repair, an exonuclease is the first enzyme involved in removing a single-stranded segment of DNA from the template strand none of the answer is correct during mismatch repair, a methylated guanine in the consensus sequence GATC found on the parent strand activates the Mut complex the Okazaki fragments synthesized during base excision repair are synthesized in a 3’ -- > 5’ fashion repair of methylated DNA requires a...
Please answer the following Case analysis questions 1-How is New Balance performing compared to its primary...
Please answer the following Case analysis questions 1-How is New Balance performing compared to its primary rivals? How will the acquisition of Reebok by Adidas impact the structure of the athletic shoe industry? Is this likely to be favorable or unfavorable for New Balance? 2- What issues does New Balance management need to address? 3-What recommendations would you make to New Balance Management? What does New Balance need to do to continue to be successful? Should management continue to invest...
A school psychologist is interested in whether time has changed the problem with student parking that has been discussed for a few years.
A school psychologist is interested in whether time has changed the problem with student parking that has been discussed for a few years. As the student population has grown the psychologist fully suspects that the time to find parking has become worse rather than better for the student body. Over the past decade the average amount of time to find parking for students is 15 minutes (µ=15). The current sample’s information is as follows: M=19 minutes and a S= 6...
The airline negotiations: A potential joint venture that did not get off the ground The grand...
The airline negotiations: A potential joint venture that did not get off the ground The grand vision The context for this case study was that the airline industry was seeking to recover from 9/11, the Sars outbreak and general uncertainty surrounding the Middle East. One industry response to these challenges was to look for opportunities to rationalise and grow through alliances. European airline was seeking to develop a position in the growing China market. At the same time, an Asian...
1. When constructing a confidence interval to estimate a population proportion, what affects the size of...
1. When constructing a confidence interval to estimate a population proportion, what affects the size of the margin of error? A. The sample size B. The sample proportion C. The confidence level D. All of the above affect the size of the margin of error E. None of the above affect the size of the margin of error 2. What percentage of couples meet through online dating apps? A survey of a random sample of couples finds that 12% say...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT