Question

If the agent causing the Missouri outbreak were not a virus related to either the Pennsylvania or the condit Alabama strain, how would your results differ in terms of particle isolation, amplification, and gel results?

Answer 1

Particle isolation: 1. isolate the virus from the infected patient's blood in lab.

2. Extract the DNA.

The amount of extracted DNA will be less to carry out further analysis hence it is suppose to be amplified.

Amplification: polymerase chain reaction (PCR) using the known primers of the pennysylvania strain and condit alabama strain

Amplification didn't happen since the primers couldn't bind to the missouri (unknown strain)

Agarose gel electrophoresis is carried out to separate the DNA strands according to their size, molecular weight, charge.

As controls pennyslvania strain and condit alabama strain are added in the well 1 and 2 respectively.

Missouri strain is added into 3rd well.

Results of gel separation: banding pattern of the Missouri strain will not be similar to both the controls indicating that the Missouri strain is not related either of the known viruses.