Question

What approaches overcame the challenge of repetitive sequences in the human genome project?

	a.	use of libraries with large genomic DNA pieces in bacterial artificial chromosomes subdivided into small subclones
	b.	mapping and alignment of restriction sites for each assembled BAC clone with human genome physical map
	c.	they removed the repetitive sequences by hybridization before sequencing
	d.	A and B
	e.	A, B, and C

Answer 1

The correct answer is : d - Both A and B are correct.

Human Genome Project or HGP is a massive project that had one very crucial challenge that created false sequencing results. The repetitive sequences present in the genome caused great difficulty in properly studying and sequencing the entire genome. To overcome this challenge, they used various next generation sequencing methods where they used BAC and created libraries of the DNA and created small subclones that could be indivdually studied while omiting the repetitive sequences