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8. 1µg of lambda DNA is digested with HindIII. This is then electrophoresed. How much DNA...

8. 1µg of lambda DNA is digested with HindIII. This is then electrophoresed. How much DNA (by weight) is present in the 23.1 kb band? How much DNA (by weight) is present in the 564 bp band? How many moles of DNA are present in each of these bands?

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The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for apps HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size. approximating the mass of DNA in comparably intense samples of similar size.

Fragment Mass (ng) bp
1 477

23,13

12 564

The average weight of a single DNA base pair (bp) is 650 daltons

In 23.1 Kb band

6.022×1023 = Avogadro’s number

The number of moles present is

ngx 6.022X1023/lengthx109X650

6.023x1014/23.1x1000x650

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