Question

How could have E. coli be used to aid in the cloning of the bioluminescence genes from Vibrio fischeri?
Gene fragments from E. coli were used to disrupt the bioluminescence genes of V. fischeri by homologous recombination.
Gene fragments from V. fischeri were cloned into plasmids, transformed into E. coli, and then retransformed back into V. fischeri.
Transposons from E. coli were used to mutagenize the bioluminescence genes of V. fischeri.
Gene fragments from V. fischeri were transformed into E. coli and bioluminescent E. coli were identified.

Answer 1

ANSWER :- d.) gene fragments of V.fischeri were transforned into E.coli and bioluminescent E.coli were identified.

The bacteria Vibrio fischeri aremotile, gram negative rod shaped cells that has the ability to produce bioluminescence and was controlled by lux operon, that consist genes that encode for enzyme luciferase (luxAB) and enzyme luxCDE that convert compound in to oxidizable subsrate.

These genes as show bioluminscence will be cloned by using the technique recombinant DNA technology (RDT), so, for that the isolated gene fragments ( Encoding LUX genes ), from V. fischeri were transformed in to E.coli using the plasmid ( extracellular circular DNA ), the recombinant gene ( or cloned genes )  is being identified in to the E.coli cells by bioluminescent.