Question

Which among the following is NOT important for PCR primer designing? A. Whole genome sequence B....

  1. Which among the following is NOT important for PCR primer designing?

A.

Whole genome sequence

B.

Protein

C.

mRNA

D.

Primer 3
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Homework Answers

Answer #1

(C) mRNA is not required for primer designing.

PCR primers are short single-stranded DNA sequences which help in the amplification of DNA during Polymerase Chain Reaction. It provides a starting point for DNA synthesis.

The key to the PCR lies in the design of two oligonucleotide primers.

  • For this information has to be sequenced using large strech of DNA stored in computer base.(hence (a) whole genome sequence is required for genome sequencing held in gene databases)
  • A number of software developments have allowed the process of primer design to be straight forward. Example (d)Primer3
  • For primers synthesis, Primase enzyme is required which is a heterodimeric protein structure. Hence (B) protein is required.
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