Question

Where are the cleavage sites by trypsin, chymotrypsin, and CNBr

Answer 1

Both Trypsin and Chymotrypsin are chemical enzymes, while CNBr is a chemical agent. All of them acts as proteases.

The cleavage sites are-

a. trypsin-- It targets the amino acids such as Lysine and arginine. And then cuts onto the C terminal side of these amino acids.

b. Chymotrypsin- On the other hand, chymotrypsin attacks the amino acids such as- Phenylalanine, Tryptophan and tyrosine. It then catalyses its cleavage action onto the C terminal sites of these amino acid residues.

c. CNBr- In protein chemistry, CNBr is being used as a highly specific protein cutter. It produces very specific cut at the C terminal of only the Methionine residues.

The common of all these three is- they all produce cuts in the c-terminal of the amino acids.