name all media that should have been inoculated to ID your unkonwn bacteria and why
Solution
|
Media |
Uses |
| · Alkaline Peptone Water | Enrichment media for Vibrio cholerae |
| · Alkaline Salt Transport Medium
· Taurocholate Peptone Transport Medium |
Transport media for diarrheal diseases suspected of being caused by V. cholerae |
| · Anaerobic Media | Liquid media by addition of
· Glucose (0.5 % to 1 %) · Ascorbic Acid (0.1 %) · Cysteine (0.1 %) · Sodium Merceptoacetate (0.1 %) · Thioglycollate (0.1 %) · Particles of cooked meat broth |
| · Bile Salt Agar
· Thiosulphate Citrate Bile Salts-Sucrose Agar (TCBS) · Monsur’s Tellurite Taurocholate Gelatin Agar |
Selective media for V. cholerae |
| · Bile Esculin Agar (Contains 40% Bile) | Selective media for Enterococcus species (Black coloration of the medium) |
| · Blood Agar | · Enriched media (Supports the growth
of fastidious organisms, e.g. Streptococcus)
· Indicator media to show hemolytic properties of certain organisms (Staphylococcus aureus: β-Hemolytic; Streptococcus pneumonia and S viridans: α-Hemolytic; Enterococcus: Non-Hemolytic) |
| · Bordet-Gengou Agar
· Charcoal Blood Agar · Regan-Lowe Medium (Charcoal Agar with blood, cephalexin and Amphotericin B) |
Isolation of Bordetella pertussis |
| · Brain Heart Infusion Broth | Used in blood culture bottles (both adult and pediatric patients) |
| · Buffered Charcoal Yeast Agar (BCYA)
· Feeley Gorman Agar |
Specialized media for isolation of Legionella |
| · Campylobacter Thioglycollate Broth | Selective holding media for recovery of Campylobacter species |
| · Castaneda Medium | Biphasic medium for the isolation of Brucella |
| · Cefoxitin Cycloserine Fructose Agar (CCFA)
· Cefoxitin Cycloserine Egg-Yolk Agar (CCEY) |
Selective media for isolation of Clostridium difficile form suspected cases of pseudomembranous colitis / antibiotic -associated diarrhea |
| · Cefsulodin-Irgasan-Novobiocin medium (CIN Medium) | Selective media for Yersinia (and may be used for Aeromonas also) |
| · Columbia-Colistin Nalidixic Acid agar (CNA Agar) | Selective media for the isolation of Gram-positive cocci |
| · Cooked meat broth
· Nutrient agar slopes · Semisolid nutrient agar stabs · Heated blood agar slopes |
In general, used for preservation and storage of bacterial cultures |
| · Crystal violet blood agar | Selective media for Streptococcus pyogenes |
| · Cysteine Lactose Electrolyte Deficient Media (CLED Media) | Most commonly used media for culturing urine samples |
| · Egg Saline Medium | Preservation of cultures of Gram-negative bacilli |
| · Egg Yolk Agar | Detection of lipase and lecithinase activity of Clostridium species |
| · Ellner’s Medium
· Medium of Duncan and Strong · Medium of Phillips · Alkaline Egg Medium |
Specialized media to induce sporulation in Clostridium |
| · Fildes Blood-Digest Agar and Broth
· Levinthal’s Agar |
Enriched media for recovery of Haemophilus influenzae |
| · Firm Agar (4% to 6% Agar) | Prevents swarming of Proteus mirabilis, P. vulgaris and Clostridium tetani |
| · Fletcher’s Agar
· Ellinghausen and McCullough Medium · Ellinghausen-McCullough-Johnson-Harris (EMJH) Media |
Solid media for isolation of Leptospira |
| · Glycerol Saline Transport Medium | Transport stool specimen for typhoid bacilli |
| · Heated Blood Agar/Chocolate Agar | Growth of fastidious organisms (E.g. Hemophilus influenzae, Neisseria gonorrhoeae and S. pneumoniae |
| · Hoyle’s Tellurite Lysed Blood Agar
· Tinsdale Medium |
Selective media for isolation of Corynebacterium from throat swabs |
| · Loeffler Serum Slope | Stimulation of metachromatic granules in Corynebacterium diphtheriae |
| · Lowenstein-Jensen Medium
· Middlebrook Media |
Selective media for isolation of Mycobacterium tuberculosis from sputum and other samples |
| · MacConkey Agar | Differential media for Enterobacteriacaeae (i.e., lactose fermenting and non-lactose fermenting) |
| · MacConkey Bile Salt Lactose Agar
· Brilliant MacConkey Agar · Leifson’s Deoxycholate-Citrate Agar (DCA) · Wilson and Blair’s Brilliant Green Bismuth- Sulphite Agar (BBSA) · Taylor’s Xylose Lysine Deoxycholate Agar (XLD) · Hektoen Enteric Agar · Salmonella–Shigella Agar |
Differential and media for isolation of Salmonella and Shigella from stool specimens |
| · Mannitol Salt Agar | Selective and indicator media for S. aureus |
| · Modified Barbour Stoenner Kelly medium (BSK) | Specialized media for Borrelia burgdorferi |
| · Modified Korthoff’s Medium | Liquid media for isolation of Leptospira |
| · Modified New York City Medium (contains colistin, lincomycin, trimethoprim, amphotericin B) | Selective media for Neisseria gonorrhoeae |
| · Mueller-Hinton Agar | Performing antimicrobial susceptibility for bacteria |
| · Nutrient Agar (1% to 2% Agar) | Basal media in microbiology
Supports the growth of all non-fastidious organisms |
| · Non-Nutrient Agar | Cultivation of parasites (e.g., Acanthamoeba) |
| · Peptone Water | · Basal media for preparation for
carbohydrate fermentation media
· To ascertain whether a bacteria is motile or non-motile · Basis for Indole test |
| · Phenol-Red Egg Yolk Polymyxin Agar | Selective media for isolation of Bacillus cereus from food, feces, and vomitus |
| · Pike’s Media | Preservation of S. pyogenes, pneumococci, and Hemophilus influenzae in nose and throat swabs |
| · Polymyxin B-lysozyme-EDTA-Thallous Acetate (PLET) | Selective media for isolation of Bacillus anthracis from soil and other medium materials containing numerous spore formers of other species |
| · Polymyxin B, Neomycin, Fusidic Acid Media (PNF) | Selective media for S. pyogenes (or β-hemolytic Streptococcus) |
| · PPLO Medium (Contains Sterol) | Specialized media for Mycoplasma pneumoniae |
| · Pre-Reduced Anaerobically Sterilized (PRAS) Media | Commercially available media for anaerobic organisms |
| · Proteose Peptone-Yeast Extraction Broth | Media for carrying out biochemical tests for anaerobes |
| · Requirements of X and V Factors | Isolation of Haemophilus influenzae |
| · Roswell Park Memorial Institute (RPMI) 1640 Medium | Cultivation of malarial parasites (i.e., Plasmodium) |
| · Robertson Cooked Meat Broth (RCMB) | · Growth of anaerobes (e.g.,
Clostridium)
· Maintaining stock cultures of anaerobic organisms |
| · Salt-Cooked Meat Broth (Cooked Meat Broth with 10% NaCl) | Enrichment media for isolation of S. aureus from heavily contaminated materials |
| · Semisolid Agar (0.05% to 0.1% Agar) | Prevents convection current and allows the growth of anaerobic and micro-aerophilic organisms |
| · Skirrow’s Campylobacter Medium (contains
polymixin B, trimethoprim, vancomycin)
· Preston Campylobacter Medium (contains polymixin B, rifampicin, trimethoprim) · Campy Blood Agar · CVA Medium (contains cefoperazone, vancomycin, amphotericin) |
Selective media for Campylobacter jejuni |
| · Smith-Noguchi Medium | Cultivation of nonpathogenic treponemes (e.g., Reiter strain of Treponema phagedenis) |
| · Sorbitol MacConkey Agar | Isolation of verocytotoxin-producing (enterohemorrhagic) E. coli of 0157 type (as it fails to ferment D-sorbitol) |
| · Stuart Transport Media
· Amies Transport Media |
Maintaining the viability of gonococci on swabs during transportation |
| · Tetrathionate Broth
· Gram-Negative Broth · Selenite-F Broth |
Enrichment media for isolation of Shigella and Salmonella from stool samples |
| · Thayer-Martin Medium (contains vancomycin, colistin, nystatin) | Selective media for Neisseria gonorrhoeae |
| · Thioglycollate Broth
· Trypticase Soy Broth |
All purpose enrichment broth for anaerobes, aerobes, micro-aerophilic, and fastidious organisms |
| · Todd Hewitt Broth with Antibiotics | Selective and enrichment for Streptococccus agalactiae in female genital specimens |
| · Triple Sugar Iron Agar (TSI) Medium | Differentiation of various members of Enterobacteriaceae |
| · Wilkins-Chalgren Agar | Performing antimicrobial susceptibility of anaerobic bacteria |
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