Question

The enzyme ABC exists in two different forms, one form is present only in the mitochondria...

The enzyme ABC exists in two different forms, one form is present only in the mitochondria and the other form can only be found in the cytosol. You are asked to purify the cytosolic ABC. Explain how centrifugation might be involved in the process.

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Density gradient centrifugation can be used to purify the cytosolic ABC enzyme.

Sequencial centrifugation can be done to fraction out some cell part in each centrifugation. The order of pallet formation in sequencial centrifugation will be : nuclei sediments, dense cell organelles like mitochondria; lysosome: peroxisomes sediments, plama membrane; ER and at last the pallet of ribosomes.

In the supernatant of last centrifugation, there will be presence of cytosolic proteins which will contain ABC enzyme.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
In a study of how external clues influence performance, university professors gave two different forms of...
In a study of how external clues influence performance, university professors gave two different forms of a midterm tests to a large group of introductory students. The questions on the test were identical and in the same order, but one test was printed on blue paper and the other on red paper (Teaching Psychology, May 1998). Grading only the difficult questions on the test, the researchers found that scores on the blue test had a distribution with a mean of...
Two p orbitals can overlap to form a σ* molecular orbital. How many nodes are present...
Two p orbitals can overlap to form a σ* molecular orbital. How many nodes are present in this σ* molecular orbital? *Is it one or three nodes? I think it’s one node. Please explain.
Troy wants to form a portfolio of four different stocks. Summary data on the four stocks...
Troy wants to form a portfolio of four different stocks. Summary data on the four stocks appears below. The average standard deviation (found simply by summing the standard deviations and dividing by 4 which is the same as the weighted average in this example) across the four stocks is 17.25%. If Troy forms a portfolio by investing 25% of his money in each of the stocks in the table, it is very likely that the standard deviation of this portfolio’s...
1) Mitochondrial DNA is a) maternally inherited b) paternally inherited c) responsible for multiple sclerosis d)...
1) Mitochondrial DNA is a) maternally inherited b) paternally inherited c) responsible for multiple sclerosis d) 10 times less likely to mutate than nuclear DNA 2) All of the following statements about the enzymic complex that carries out the synthesis of ATP during oxidative phosphorylation are correct EXCEPT: A. It is located on the matrix side of the inner mitochondrial membrane. B. It is inhibited by oligomycin. C. It contains a proton channel. D. It can exhibit ATPase activity. E....
1) You have two unknown, unlabeled clear solutions. One is DNA and the other is protein....
1) You have two unknown, unlabeled clear solutions. One is DNA and the other is protein. How do you determine which solution is DNA, which one is protein 2)In an SDS gel larger proteins travel ________________(longer/shorter) distance compared to smaller proteins. 3) Assuming that the isolation and purification of Rubisco went as expected, which fraction isolated should have the highest concentration of pure Rubisco? Please explain. (There was a total of 11 fractions) 4) Your first task as a graduate...
1.Suppose that two proteins, A and B, can associate with each other to form a complex.  ...
1.Suppose that two proteins, A and B, can associate with each other to form a complex.   You measure the dissociation constant at several different temperatures and find that as you heat up the sample, the Kd decreases. Does this mean that binding of A and B becomes more favorable or less favorable as the temperature increases? 2.Calculate the ΔG for ATP hydrolysis in a cell in which the [ATP]/[ADP] ratio had climbed to 100_1, while the Pi concentration remained at...
Sometimes two studies can reach opposite conclusions, BUT not necessarily be contradictory. For example, one might...
Sometimes two studies can reach opposite conclusions, BUT not necessarily be contradictory. For example, one might conclude that there is no relation between frustration and aggression; another might conclude there is. Apply the concept of operational definition in a simple example that illustrates how such differences may be only artifacts of measurement and not really different afterall. You may use frustration and aggression or something else.
1. What is an industry that has only one significant supplier? What is the nature of...
1. What is an industry that has only one significant supplier? What is the nature of the product? How easy would it be to start a similar firm or stop offering the product/service? How easy is it to attain information about the industry or product or service? 2. Are there any products that are sold in a market in which the seller is able to charge different prices to different consumers? How are the sellers able to do this? 3....
You’ve got two lottery tickets available to you. One of them offers 30 different REPEATING(same number...
You’ve got two lottery tickets available to you. One of them offers 30 different REPEATING(same number can be pulled multiple times) numbers for you to pick FOUR from. The other offers 34 different NON-REPEATING(the number is removed from the pool of numbers) numbers for you to pick FIVE from. Calculate the odds for winning on each of these lottery tickets (assume there’s only ONE winning combination for each of them), write them down, then pick the one which gives you...
The presence of an impurity can affect the observed melting point of a sample in two...
The presence of an impurity can affect the observed melting point of a sample in two different ways: certain impurities can have only one, only the other, or both effects depending on what the impurity is and how much is present. What are the two effects (that is, how would the observed melting range change compared to the literature value of the pure substance)? a. b. One measures the amounts of lots of chemicals in a reaction, including those that...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT