Question

In your studies of neurotransmitter release, you accidentally raise your neurons in a calcium-free medium. To nobody’s surprise, they do not release neurotransmitter. After switching the cells to a calcium-containing medium, they still do not release neurotransmitter when stimulated. After going through your notes, you see that your lab partner had ordered mutant cells. A) Explain what the mutation might have been, and B) how you would test that hypothesis (ie, what would you do to allow the cells to release neurotransmitter – assuming that the protein from A was the only defect).

Answer 1

Mutation is the any change of genitic constution of an organism.It may caused by different reasons.The causative factors called mutagens.According to the factors which affecting to mutations classified into different types mainly three types.

       A.In this situation neurons put in a condition,that means calcium free meadium.the neurotransmitters are the substance which help to transmit the impulses throuh synapse.it may nonsense mutation ,the absence of calcium may cause tolter the dna sequence and stop the production of particular protein.

B. Normally we can do the protein analysis.If we have to a high level accurency deals with DNA finger print technology