Question

All of the following are associated with rho-independent termination of transcription, except:

		Three characteristic uracil residues near the 3' end of the transcript
		A shortened DNA-RNA hybrid that leads to transcript dissociation
		Helicase binding to a CA-rich element
		Involvement of a hairpin RNA structure in termination

Answer 1

Ans:

Helicase binding to a CA-rich element

Explanation: In rho-independent termination of transcription, rho-factor not needed for termination. In this type of termination, transcripts form a G-C rich harpin loop structure followed by U- rich region and the hairpin distabilized the DNA-RNA hybrid that leads to dessociation of the RNA from DNA. No helicase enzyme is needed.

In rho-dependent termination of transcription,  rho-factor is needed for termination which is a ATP-dependent RNA-stimulated helicase that binds a C-A rich site called rut site.